European Journal of Biotechnology and Bioscience

Authors
M.S. Shinkafi, I.U. Mohammed, A.A. Audu

Abstract
Microorganisms have potential ability to decolorize synthetic commercial dyes used for textile dyeing.rnEffluents from textile and dyeing industries cause serious pollution to air, soil and water environment.rnTherefore, this study was aimed at isolating potential dye degrading microorganisms from textilerneffluents and to evaluate their capability to decolorize commercially used textile dyes. Effluent samplernwere collected from the confluent of the textile wastewater discharge of African Textile Company inrnKano, Nigeria. Two candidates’ microorganisms (Pseudomonas putida and Klebsiella ozaenae) werernused to decolorize and degrade dyes into non-toxic form. This same isolates were mutated using UVirradiationrninducement and resulted into the wild and mutant types of bacterial isolates. Cultures werernmade in thirteen (13) reaction flasks, each containing the same concentration of two different textilerndyes; Congo red (Direct red 28) and Direct blue ‘80’. Degradation ability of dyestuffs by the isolatesrnwas observed by dye decolorization assay. The reactive capacity of dye Congo red and direct dye 80rnwere determined spectrophotometrically by monitoring the absorbance of different dyes at constantrnwavelength (λmax). Virtually all the isolates were able to decolorize the dyes considerably afterrnspecified periods of incubation.