Nitin N. Bolabattin, Yogesh N. Joshi, Vinod P. Sinoorkar
The Bee venom is used for treating a wide variety of conditions from acute tendonitis to chronic back pain to rheumatoid arthritis (RA) from ancient times. The use of honey bee products for medical purposes, this includes bee venom, raw honey, royal jelly, pollen, propolis, and beeswax, is called as apitherapy. Bee venom (apitoxin) is an active toxic substance, composed of only a few pharmacologically and enzymatically active components (18 active componenets), such as phospholipase A, hyaluronidase, histamines, melittin & apamin, a mast cell degranulating peptide (MCD). Melittin is one of the abundant active component of Bee venom (52% of bee venom) with biologically positive effects (wide antimicrobial, anti-inflammatory, hemolytic, cytolytic) having low toxicity which can be used to treat many diseases. The present investigation includes retrieval of amino acid information of melittin from major protein sequence database, sequence analysis of melittin was performed using Protoparam tool. The physiochemical parameters like amino acid propensity, molecular weight, isoelectric point, aliphatic index, hydropathicity were determined. The secondary structure of melittin was predicted using GOR method. The antigenecity region or peptide was predicted by using Kolaskar & Tongoankar method. Further in silico characterization of structural and functional elements of melittin can put insight into the new pharmacological applications of apitoxins emerging from oldest form of traditional medicines.