European Journal of Biotechnology and Bioscience

Authors
Anushree, Gopi A, Girisha ST, Devaraju KS

Abstract
DNA is known to be cleaved by nitration process, other than endonucleases. Here in this study, it has been found that DNA undergoes both non-enzymatic by peroxynitrite and enzymatic degradation by restriction endonucleases (EcoRI, BamHI, HindIII and NdeI). This is due to the nitration process on guanine residues makes DNA susceptible for the hydrolytic cleavage. It has been found that DNA damage due to peroxynitrite treatment is mostly depend on the presence of guanine and hence DNA damage decreases in the order of BamHI (5’- GGATCC-3’)> EcoRI (5’-GAATTC-3’)> HindIII (5’- AAGCTT-3’)> NdeI (5’-CATATG-3’). Further it has been found that the UV Visible characteristics of nitrated product do not changes. The nitration process increases as the concentration of peroxynitrite being increased. It means that, DNA undergoes hydrolytic cleavage and hydrolytic product will remain stable. So to neutralize these nitration products further studies need to be done.