European Journal of Biotechnology and Bioscience

European Journal of Biotechnology and Bioscience


European Journal of Biotechnology and Bioscience
European Journal of Biotechnology and Bioscience
Vol. 4, Issue 10 (2016)

In- silico Structural & Molecular characterization of Pectin methylesterase from Arabidopsis thaliana


Yogesh N Joshi, Prathusha S Chegu, Nitin N Bolabattin, Chetan Godale

Pectin methylesterase (PME) is a ubiquitous cellwall- associated enzyme present in plants & certain micro-organisms. PME is a hydrolytic enzyme that catalyzes demethylesterification of the pectin homogalacturonan which present in the plant cell wall producing pectate and methanol. It has been proved that PME enzyme plays important role in seed germination of Arabidopsis thaliana allowing water uptake. The present in-silico study includes retrieval of sequence of PME of A. thaliana & its analysis followed by prediction of its domain, secondary structure & 3D structure which is further validated by PROCHECK analysis using various Insilco tools & servers. The results showed that the PME is alkaline, cationic enzyme having two domains PMEI & pectin esterase. In the secondary structure, it contains more % of random coils than helices & sheets & the most favorable region of predicted 3D structure is 86.1%. These findings will put insight into its functional role in seed germination which will be helpful to find the natural inhibitor & also to design the novel inhibitor to stop the seed germination process.
Download  |  Pages : 21-24
How to cite this article:
Yogesh N Joshi, Prathusha S Chegu, Nitin N Bolabattin, Chetan Godale. In- silico Structural & Molecular characterization of Pectin methylesterase from Arabidopsis thaliana. European Journal of Biotechnology and Bioscience, Volume 4, Issue 10, 2016, Pages 21-24
European Journal of Biotechnology and Bioscience