European Journal of Biotechnology and Bioscience

European Journal of Biotechnology and Bioscience


European Journal of Biotechnology and Bioscience
European Journal of Biotechnology and Bioscience
Vol. 4, Issue 10 (2016)

Optimum conditions for L-asparaginase production by Rhodococcus erythropolis VLK-12 isolated from Marine habitats


Krishna Naragani, Vijayalakshmi Muvva

The aim of present study includes thernproduction and optimization of L-asparaginase from Rhodococcus erythropolisrnVLK-12 isolated from marine soil samples of Visakhapatnam, Andhra Pradesh.rnAmong the 20 actinobacterial strains screened for enzyme production, onernisolate found to be potent was identified as Rhodococcus erythropolisrnVLK-12 using morphological, cultural, physiological, biochemicalrncharacteristics and 16S rRNA analysis. Attempts were made to optimize the culturalrnconditions affecting the production of L-asparaginase by the strain. Maximalrnyields of L-asparaginase were recorded from 3-day-old culture grown in modifiedrnasparagine-glycerol salts broth (ISP-5) with initial pH 7.0 at temperature 30oC.
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How to cite this article:
Krishna Naragani, Vijayalakshmi Muvva. Optimum conditions for L-asparaginase production by Rhodococcus erythropolis VLK-12 isolated from Marine habitats. European Journal of Biotechnology and Bioscience, Volume 4, Issue 10, 2016, Pages 01-05
European Journal of Biotechnology and Bioscience