European Journal of Biotechnology and Bioscience

Authors
Krishna Naragani, Vijayalakshmi Muvva

Abstract
The aim of present study includes thernproduction and optimization of L-asparaginase from Rhodococcus erythropolisrnVLK-12 isolated from marine soil samples of Visakhapatnam, Andhra Pradesh.rnAmong the 20 actinobacterial strains screened for enzyme production, onernisolate found to be potent was identified as Rhodococcus erythropolisrnVLK-12 using morphological, cultural, physiological, biochemicalrncharacteristics and 16S rRNA analysis. Attempts were made to optimize the culturalrnconditions affecting the production of L-asparaginase by the strain. Maximalrnyields of L-asparaginase were recorded from 3-day-old culture grown in modifiedrnasparagine-glycerol salts broth (ISP-5) with initial pH 7.0 at temperature 30^oC.