European Journal of Biotechnology and Bioscience

Authors
Massinissa Yahia, Mouloud Yahia, Afaf Benhouda, Nassiba Chafaa, Hachani Khadraoui, Djahida Benhouda, Boutaghrine Sihem

Abstract
Objective: The goal of this present work is to evaluate the anti-hepatotoxicity activity in vivo of the methanolic extract of Hyoscyamus albus’ leaves (HAMeOH) on Wistar rats against CCl4 induced liver damage. Methods: The animals were divided into seven groups with five rats in each group. CCl4 (2 mL Kg^-1 b.w.) was injected subcutaneously in the second and the third day of the experiment, and the methanolic extracts of HAMeOH (100 and 200 mg/ kg b.w.) was given by gavage fifth day of the experiment. The extracts prevented liver damage caused by CCl4, as noted by the significant decrease in serum aminotransferases release and the alkaline phosphatase activities. This extracts decrease the necrosis effect of CCl4 in the histological examination. Results: Serum ALAT and ASAT and ALP activities significantly decreased in a dose-dependent manner in treatment groups with extracts. Histological examination showed liver damage in HAMeOH-treated groups lower.