European Journal of Biotechnology and Bioscience

Authors
Esin Poyrazoğlu Çoban, Halil Bıyık, Bülent Bozdoğan

Abstract
The aim of the study was to determine genetic characterization of acetic acid bacteria producing the bacterial cellulose (BC) and increase the amount of production by genetic modification to be applied. Acetic acid bacteria were isolated from home-made vinegar and examined cellulose production yields. Acetic acid bacteria which product high yields of cellulose were determined as Gluconacetobacter hansenii. DNA isolation, plasmid extraction and amplification of DNA fragments were carried out for gene scans of these bacteria. All of genome sequence was made by Macrogen Inc. (Korea). But, the whole genome sequence wasn’t obtained in the analysis performed and instead, sequences of DNA fragments on various sizes (20 bp - 150 kb) called contig could be identified. Mutant strains were obtained by UV application. It was confirmed that mutant strains produced high yield cellulose.