European Journal of Biotechnology and Bioscience

Authors
Ellappan Kalaiarasan, Harish Belgode Narasimha

Abstract
Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic and nosocomial pathogen which causes severe life threatening infections worldwide. The present study was carried out to analyze the antimicrobial resistance patterns, hemolysin and pigments production among biofilm forming P. aeruginosa. A total of 50 biofilm forming P. aeruginosa isolates were included in this study. During Antimicrobial susceptibility testing (AST), ceftazidime resistance was observed in 46% of biofilm producing isolates, amikacin (42%), gentamicin (34%), ciprofloxacin (26%) and meropenem (12%). Using conventional PCR assay, the studied isolates were speciated by species specific primers of P. aeruginosa. The biofilm assay was carried out using 96 well micro titer plates and Muller Hinton agar plate was used to detect the pigments production. In addition, pyocyanin assay was conducted using chloroform extraction method. Hemolytic activity was accessed by using sheep blood agar plate. During PCR analysis, all the studied isolates were belongs to P. aeruginosa. Among biofilm forming isolates, pyoverdine, pyocyanin and pyorubin production was detected in 42%, 14% and 4% of isolates, respectively. Hemolytic was observed in 48% of biofilm forming isolates. Our study indicates that the involvement of antimicrobial resistance and virulence factors in biofilm producing P. aeruginosa remains a challenge for clinicians to provide treatment against such infections.