Acute toxicity and indirect effects on the NA+/K+ ATPase pump of chloroform extract of Mansonia altissima (Sterculiaceae) in rabbits
Mansour Franck Adeoti, Philippe Bidie, Massara Camara-Cisse, Gnate Francois Onteomo, Kouamé Innocent Kolia, Konan Gogahy, Allico Joseph Djaman, Mireille Dosso
Mansonia altissima bark is known as poison but also for these multiple effects including cardio- protective effect. This study determine to acute toxicity and effect on the Na+/K+ of chloroform extract of Mansonia altissima bark. Acute toxicity of chloroform extract of Mansonia altissima was conducted. Then, ion pool was performed by administrating increasing doses (4.7-75 mg/kg) of chloroform extract in 36 rabbits devised in 7 lots and ion (Ca2+, Mg2+, Na+ and K+) were determinate by spectrophotometer analyzer. The results showed a lethal lose 100 (DL100) of 125 mg/Kg, lethal lose 50 (LD50) of 92.5 mg/Kg and maximum tolerated dose (MTD) of 75 mg/Kg in mice type albino. The value of LD50 indicates that chloroform extract has moderate toxicity. Results of ion pool showed significant (p<0.05) decrease of Na+ and non-significant Ca2+ concentrations in plasma, associated with non-significant first increase (p<0.01) in plasma of K+ concentration for lower doses of extract at 18.7 mg/kg of body mass weight. Plasma concentrations of Mg2+ were also experienced a non-significant increase (p <0.10) in relation to the pump Na+/K+ ATPase inactivation. These results relate that administration of non-toxic doses of chloroform extract of Mansonia altissima bark inhibit action on Na+/K+ ATPase and myocardial contraction.
Mansour Franck Adeoti, Philippe Bidie, Massara Camara-Cisse, Gnate Francois Onteomo, Kouamé Innocent Kolia, Konan Gogahy, Allico Joseph Djaman, Mireille Dosso. Acute toxicity and indirect effects on the NA+/K+ ATPase pump of chloroform extract of Mansonia altissima (Sterculiaceae) in rabbits. European Journal of Biotechnology and Bioscience, Volume 4, Issue 11, 2016, Pages 29-33