European Journal of Biotechnology and Bioscience

Authors
Mansour Franck Adeoti, Philippe Bidie, Massara Camara-Cisse, Gnate Francois Onteomo, Kouamé Innocent Kolia, Konan Gogahy, Allico Joseph Djaman, Mireille Dosso

Abstract
Mansonia altissima bark is known as poison but also for these multiple effects including cardio- protective effect. This study determine to acute toxicity and effect on the Na⁺/K⁺ of chloroform extract of Mansonia altissima bark. Acute toxicity of chloroform extract of Mansonia altissima was conducted. Then, ion pool was performed by administrating increasing doses (4.7-75 mg/kg) of chloroform extract in 36 rabbits devised in 7 lots and ion (Ca²⁺, Mg²⁺, Na⁺ and K⁺) were determinate by spectrophotometer analyzer. The results showed a lethal lose 100 (DL₁₀₀) of 125 mg/Kg, lethal lose 50 (LD₅₀) of 92.5 mg/Kg and maximum tolerated dose (MTD) of 75 mg/Kg in mice type albino. The value of LD₅₀ indicates that chloroform extract has moderate toxicity. Results of ion pool showed significant (p<0.05) decrease of Na⁺ and non-significant Ca²⁺ concentrations in plasma, associated with non-significant first increase (p<0.01) in plasma of K⁺ concentration for lower doses of extract at 18.7 mg/kg of body mass weight. Plasma concentrations of Mg²⁺ were also experienced a non-significant increase (p <0.10) in relation to the pump Na⁺/K⁺ ATPase inactivation. These results relate that administration of non-toxic doses of chloroform extract of Mansonia altissima bark inhibit action on Na⁺/K⁺ ATPase and myocardial contraction.