European Journal of Biotechnology and Bioscience

Authors
Rashmi Verma, Mahender Singh, Sudhir Kumar, Vishwamitra Singh Baiswar

Abstract
Understanding gene flow and their effects on populations is useful in identification and selection of different breeding groups and for protecting genetically distinct and unique populations and therefore helpful in designing appropriate restocking programs and management strategies. Tenualosa ilisha commonly called as Hilsa, constitutes a sizeable fishery in both the west and east coasts of India. However, little work has been reported to differentiate various hilsa populations. In our study successful cross priming amplification was observed with thirty one primer pairs of related species out of which nine polymorphic microsatellite loci were found. The observed number of alleles ranged from 2 (Aa20) to 11 (Asa4) with a total of 311 alleles and mean of 4.9 alleles across loci. The mean expected and observed heterozygosity across loci within populations ranged from 0.5114 to 0.5669 and 0.6142 to 0.8333. Thirty out of sixty three loci were in showed significant departure from Hardy-Weinberg equilibrium (HWE) and all locus have negative FIS values. Our data suggest that the Hilsa populations have not experienced a genetic bottleneck in the recent past.