European Journal of Biotechnology and Bioscience

Authors
Ashim Chakravorty

Abstract
Present investigation aims at isolation of three somaclonal variants after screening two elite cultivars of rice landraces viz., Jhingasail and Sarkele aman regenerated from matured seed. The explants were cultured on MS medium supplemented with BAP (3.5mgl-1). Both qualitative and quantitative analyses were done on the three somaclonal variants for each cultivars developed through tissue culture method. Remarkable significant variability was observed in these variants over the plants developed through conventional breeding in respect to agro-morphological and biochemical characters. Among three somaclones of Jhingasail, J-3 performed well in respect to maximum traits and SA-3 somaclonal variant of Sarkele amon showed better variability pattern in comparison to other variants. This study would be helpful to the researchers and plant breeder for future crop improvement programme to conserve the landraces and maintain the germplasm through in vitro culture method.