European Journal of Biotechnology and Bioscience

European Journal of Biotechnology and Bioscience


European Journal of Biotechnology and Bioscience
European Journal of Biotechnology and Bioscience
Vol. 4, Issue 5 (2016)

Comparative effect of aqueous and methanol extract of Buchholzia coriacea seeds on carrageenan-induced inflammation in rats


Okere OS, Abioye JOK, Adamu TJ

This study wasrndesigned to investigate the comparative effect of aqueous and methanol extractrnof Buchholzia coriacea seeds on carrageenan-induced inflammation inrnrats. Twenty four albino rats of both sexes weighing 162.53±3.55 g were used.rnFor the aqueous extract, 12 animals were divided into 4 groups of 3 rats each.rnAnimals in group 1 (positive control) were administered 0.5 ml of diclofenacrnsodium (reference anti-inflammatory drug) while those in group 2, 3 and 4 werernadministered 100, 300 and 500 mg/kg body weight of the aqueous extract of Buchholziarncoriacea seeds respectively. For the methanol extract, 12 animals wererndivided into 4 groups of 3 rats each. Animals in group 1 (positive control)rnwere administered 0.5 ml of diclofenac sodium (reference anti-inflammatoryrndrug) while those in group 2, 3 and 4 were administered 100, 300 and 500 mg/kgrnbody weight of the methanol extract of Buchholzia coriacea seedsrnrespectively. Thirty minutes after treatment of animals in both the aqueous andrnmethanol groups with the drug and extract, 0.2 ml of 2% carrageenan wasrninjected into the left hind paw of each animal under the sub plantarrnaponeurosis. Paw volume of rats were measured before and after carrageenanrninjection at 30 minutes interval over a period of 3 hours (30, 60, 90, 120, 150rnand 180) minutes, to determine the anti-inflammatory response of both extracts,rnusing carrageenan-induced paw odema method. Preliminary phytochemical screeningrnrevealed that the aqueous extract contains terpenoids, flavonoids and steroidsrnwhile the methanol extract contains saponins, flavonoids, tannins,rnanthraquinones, cardiac glycosides, terpenoids and steroids. Proximate analysisrnof Buchholzia coriacea seeds revealed that crude fibre has the lowestrnpercentage of (1.10±0.05), ash content (5.20±0.01), crude fat (4.10±0.02)rnmoisture content (9.10±0.13), crude protein (10.20±0.20) and carbohydrate withrnthe highest percentage of (70.10±0.04). In terms of percentage inhibition, pawrnvolume was brought to near normal levels by the methanol extract at 100 and 300rnmg/kg body weight which showed better anti-inflammatory activity than 500 mg/kgrnbody weight of the same extract as well as all doses of the aqueous extract andrneven more than the reference drug. Overall, the anti-inflammatory effect may berntraced to single or synergistic activity of phytoconstituents present in thernmethanol extract.
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How to cite this article:
Okere OS, Abioye JOK, Adamu TJ. Comparative effect of aqueous and methanol extract of Buchholzia coriacea seeds on carrageenan-induced inflammation in rats. European Journal of Biotechnology and Bioscience, Volume 4, Issue 5, 2016, Pages 01-08
European Journal of Biotechnology and Bioscience