European Journal of Biotechnology and Bioscience

Authors
Khadke SG, Kothekar VS

Abstract
Winged bean (Psophocarpuste tragonolobusrnL.) belong to family Fabeaceae is a nutritious legume in human diet, itsrnmutants like the high seed yield, dwarf, early maturity and low trypsinrninhibotor content carrying would assume substantial economic importance. In thernpresent investigation these mutants of Winged bean were biochemically analyzedrnfor different parameters like crude protein content, seed soluble proteinrncontent and low trypsin inhibitor content. The viable mutants demonstrated thernseed crude protein content in the range of 38% to 54.66%. The dwarf mutantrndisplayed lowest seed protein content of 20.76%, while the highest crude seedrnprotein content could be seen in the high yielding/large leaf p-2 mutant ofrnwinged bean. The highest amount of protein content 36.33 µg/ml of defatted seedrnpowder could be seen in high yielding/large leaf p-2 type of mutant in wingedrnbean. The lowest protein content 24.66 µg/ml of defatted seed powder wasrnobservable in dwarf mutant of winged bean. The protein polymorphism ofrndifferent viable mutants revealed a wide variability with respect to the numberrnand mobility of bands. A marginal difference in TI content and iso inhibitorrnprofile was detected in micromutants of winged bean. The lowest TI (272.7rnTIU/min/gm meal) could be recorded in dwarf mutant, while the linear leafletrnmutant revealed the highest TI content (476.45 TIU/min/gm meal). Out of sevenrniso inhibitors, four iso inhibitors of trypsin were major, while three iso inhibitorsrnwere minor with weak expression as revealed by X-ray film technique.