European Journal of Biotechnology and Bioscience

European Journal of Biotechnology and Bioscience


European Journal of Biotechnology and Bioscience
European Journal of Biotechnology and Bioscience
Vol. 4, Issue 6 (2016)

Encapsulation of somatic embryoids of Curculigo orchioides Gaertn. and its germination


KS Nagesh, C Shanthamma

Curculigo orchioides Gaertn is an endangered medicinal plant with antioxidant and anticancer properties. The rhizome and tuberous roots of the plant have been used extensively in India in indigenous medicine. Due to its multiple uses, the demand for Curculigo orchioides is constantly on the rise; however, the supply is rather erratic and inadequate. Destructive harvesting, combined with habitat destruction in the form of deforestation has aggravated the problem. The plant is now considered ‘endangered’ in its natural habitat. Therefore, the need for conservation of this plant is crucial. Here we described an efficient protocol for germination of sodium alginate encapsulated embryoids of Curculigo orchioides. Morphogenic callus was induced on Murashige and Skoog (1962) [16] basal medium (MS) containing 0.5 - 3 mg/1 of 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 0.5 mg/1 N6 –benzylaminopurine (BAP) from rhizome explants. Transfer of morphogenic calli to MS medium with 1-4 mg/1 BAP resulted in somatic embryogenesis, at high frequencies with an average of 23 ± 0.8 somatic embryos per gram of morphogenic callus on MS medium with BAP (1 mg/l). Further, germination of sodium alginate encapsulated somatic embryoids of Curculigo orchioides were tested on half-strength Murashige and Skoog (MS) basal medium fortified with coconut water (10% v/v). The frequencies of regeneration from encapsulated somatic embryoids were affected significantly by concentration of sodium alginate and the duration of exposure to calcium chloride. Somatic embryoids encapsulated with 2.5% sodium alginate dissolved in MS basal salts solution recorded significantly higher germination than other treatments. A relatively short (5 min) incubation with calcium chloride solution provided uniform encapsulation of somatic embryoids that gave the highest percentage (80%) of germination. Germinated synthetic seeds produced normal plantlets. Based on the present observations an improved protocol may be developed for ex-situ conservation of the endangered species – C. orchioides.
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How to cite this article:
KS Nagesh, C Shanthamma. Encapsulation of somatic embryoids of Curculigo orchioides Gaertn. and its germination. European Journal of Biotechnology and Bioscience, Volume 4, Issue 6, 2016, Pages 38-42
European Journal of Biotechnology and Bioscience