European Journal of Biotechnology and Bioscience

European Journal of Biotechnology and Bioscience


European Journal of Biotechnology and Bioscience
European Journal of Biotechnology and Bioscience
Vol. 4, Issue 7 (2016)

Quantitative estimation of amino acids and carbohydrates in the root exudates of Eucalyptus alba and Ziziphus nummularia


Mahavi O Makadia, Nilesh S Panchal

Substances released byrnhealthy and intact roots into the environment are collectively designated asrnroot exudates. In present study, Eucalyptus alba and Ziziphusrnnummularia were selected as experimental plants. E. alba are plantedrnon a massive scale worldwide for fuel-wood, poles, timber and other woodrnproducts, it yield valuable essential oils, whilst some also provide anrnexcellent source of nectar for honey bees (flowers have been used in the beernkeeping industry for honey) while Z. nummularia provide excellent fodderrnfor livestock as well sourceas of high calorific value fuel & charcoalrnwhen dried, and leaf paste used to treat scabies and other skin diseases, it’srnheartwood is used in farm implements & for house construction, it’s barkrnhas nematicidal, antihelminthic, antipyretic and anti- inflammatory propertiesrnand it is erected as micro-windbreaks. Root exudates were collected from bothrnthe plants and carbohydrate and amino acids were measured quantitatively.rnProtein/amino acids estimation were done by the methods Ninhydrin andrnBradford’s colorimetric assay while carbohydrate estimation was done by thernmethods Anthrone and Phenol-sulfuric Acid colorimetric assay. Result of thisrnstudy showed that E. alba was showing better result than Z. nummulariarnin the amino acids quantitative assays while Z. nummularia wasrnshowing better result than E. alba in the carbohydrates quantitativernassays. In the rhizosphere, root exudate plays an important role as attractingrnsignal molecules to attract the plant growth promoting microbes (endophytes andrnrhizobacteria) which performs important plant growth promoting functionsrn(biocontrol, hormone production and bioremediation). Experimental plants showedrnthe presence carbohydrate and amino acids in root exudates, which will helprnthem in the promotion of plant growth, immunity and increase the resistance tornarid and saline conditions which is not only of economic importance but alsorncounted as contribution to the agriculture field.

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How to cite this article:
Mahavi O Makadia, Nilesh S Panchal. Quantitative estimation of amino acids and carbohydrates in the root exudates of Eucalyptus alba and Ziziphus nummularia. European Journal of Biotechnology and Bioscience, Volume 4, Issue 7, 2016, Pages 56-62
European Journal of Biotechnology and Bioscience