Micropropagation of Cyrtanthus mackenii Hook. f.: from tri scales
Sincy Joseph, Lekha Kumar, Narmatha Bai V
A procedure for in vitro production of bulbs of Cyrtanthus mackenii was developed from triscales. The explants were cultured on MS medium supplemented with cytokinins such as BAP (6-Benzyl amino purine), TDZ (Thidiazuron), Zeatin, Kinetin and auxins namely IAA (Indole-3-aceticacid), IBA (Indole-3 butyric acid), NAA (Napthalene acetic acid), individually at different concentrations. A combination of TDZ and NAA in equal concentrations (1mg/l) produced a maximum 14 bulblets. The maximum number of roots (6) root length (4.96cm) was recorded on TDZ (1mg/l) + NAA (1mg/l). Sucrose at various concentrations (30, 60, 90 and 120g/l) was tested to increase the size of bulblets. Increase in the size of bulblets (growth index) was noticed after three months at 60g/l concentration which gave the maximum weight of 4.20gms. Bulblets were then transferred to pots containing a potting mixture of vermiculite: soil (3:1). The plants showed 98% survival. Regenerated plantlets exhibited morphological characters similar to source plant.