European Journal of Biotechnology and Bioscience

European Journal of Biotechnology and Bioscience


European Journal of Biotechnology and Bioscience
European Journal of Biotechnology and Bioscience
Vol. 4, Issue 8 (2016)

Cost-effective medium for the production of mosquitocidal toxins from a novel strain Bacillus thuringiensis subsp. israelensis/tochigiensis (H14/H19)


Shankar K, Prabakaran G, Manonmani AM

An indigenous isolate of Bacillusrnthuringiensis sharing the antigens of israelensis/tochigiensisrnwas for the first time reported to exhibit mosquito larvicidal activity. Inrnthis study a cost effective medium using peanut milk was developed forrnproduction of the mosquitocidial endotoxin for this strain. Shake flaskrnexperiments were conducted using 2.5%, 5.0% and 7.5% of peanuts and comparedrnwith the conventional medium for biomass production, spore count andrnmosquitocidal activity. In stationary phase of growth, Peanut milk medium 5%rngave dry cell mass yield of 3.14 g/L, spore count of 1.5 x 109 CFU/mlrnand mosquito larvicidal activity (LC50) of 0.010 µg/ml against laternthird instar larvae of Culex quinquefasciatus by 18 h as againstrnthe dry cell mass yield of 2.40 g/l, spore count of 1.2 x 109 CFU/mlrnand mosquito larvicidal activity (LC50) 0.007 µg/ml obtained usingrn24 h old culture of the conventional Nutrient yeast Extract Salt Medium (NYSM).

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How to cite this article:
Shankar K, Prabakaran G, Manonmani AM. Cost-effective medium for the production of mosquitocidal toxins from a novel strain Bacillus thuringiensis subsp. israelensis/tochigiensis (H14/H19). European Journal of Biotechnology and Bioscience, Volume 4, Issue 8, 2016, Pages 12-16
European Journal of Biotechnology and Bioscience