Tolerance of biofilm forming bacteria to thermal shock and detergent
Rupal Singh, Abin Mani, Vijay Thawani
Biofilm forming bacteria play a vital role inrnecological balance. The objective of this study was to determine the effect ofrnthermal shock and detergents on the survival of biofilm forming bacteria. The Escherichiarncoli biofilm were subjected to thermal shocks in the range of 60°C to 100°Crnfor duration between 5 to 60 min. Effect of different concentrations (1-10%) ofrnSodium Dodecyl Sulfate (SDS) on survival of bacteria was also studied. Colonyrnforming units (CFU) were quantified using direct enumeration method. Reductionrnof CFU concentration with increasing temperature was observed. E. colirncells were able to survive at 60°C up to 20 min duration, while at 70°C thernbacteria could not survive even for 5 min. Increasing SDS concentration alsornresulted in reduction of bacterial colonies. Biofilm forming E. colirndemonstrated tolerance to high thermal shock and detergent. This can assist inrnstabilization of anthropogenic-induced environmental imbalance.