European Journal of Biotechnology and Bioscience

Authors
Dr. Hadeel M Younis, Hiba Y Khalaf, Nahla K Asaad, Abdul Nasir

Abstract
Aspergillus is a filamentous fungus opposite to yeast which is single cells. Fungi reproduce by forming tiny spore which can easily be airborn. Some pathogenic aspergillus species can cause serious disease in humans and animals. Aspergillus can produce mycotoxins these are often found in contaminated foodstuff and are hazardous to hay and s are easily airborne and we are normally breathe in 100-200 spores daily. The most common causative invasive disease are aspergillus fumigatus and aspergillus flavus. While the most causing allergic disease are aspergillus fumigatus and aspergillus clavatus (4). Aspergillosis is a group of disease caused by aspergillus which produce symptoms includes fever, cough, chest pain, or breathlessness occur in many other illness. Laser is a device that generate light by a process called stimulated emission. The acronym laser stands for light amplification by stimulated emission of radiation. Semiconducting lasers are multilayer semiconductor devices that generates a cohere beam of monochromatic light by laser action. A coherent beam resulted which all of photons are in phase. This study was carried out to: 1) isolate and diagnose of Aspergillus spp. and 2) evaluate the effect of low-power diode laser on growth of Aspergillus spp. fifty samples were taken from skin scraping and hair. The samples were collected from human after cleaning the area with 70% ethanol to remove contaminants such as bacteria. There are three examination for analysis the specimens: direct examination (direct microscopy), culture, staining by lacto phenol cotton blue (L.P.C.B.). The present study showed that 50% of isolates are aspergillus spp from all collected clinical specimens which are mature within 3 days. Also the present study showed that decrease in number of aspergillus spp. after exposure to laser for 4, 8, 16, 32, and 64 minutes. In conclusion: Exposure low power diode laser light was inhibited the growth of Aspergillus spp. after 32, 64 minutes.