In vitro bioactivity screening of methanolic extracts of Artocarpus heterophyllus, Artocarpus altilis and Piper betle
Sindhu S Nair, Vaibhavi Kavrekar
The aim of this work was to evaluate the antimicrobial, haemagglutination, cytotoxic, and phytotoxic activity of the selected plants namely Artocarpus altilis, Piper betle and Artocarpus heterophyllus. The leaves of Artocarpus altilis and Artocarpus heterophyllus and roots of Piper betle were subjected to methanolic solvent extraction and in vitro bioactivity screening for the presence of various bioactive constituents. The haemagglutination activity of the extracts were tested with human erythrocytes of Rh + and Rh – types of the blood groups A, B, AB and O. Haemagglutination was tested using tube agglutination method. Antimicrobial screening of the methanolic plant extracts was performed by the standard disc diffusion method. The organisms used in this assay were two Gram positive strains of microorganisms namely Staphylococcus aureus, Bacillus subtilis and two Gram negative strains namely Escherichia coli, Pseudomonas aeruginosa and four fungal strains were selected for antifungal screening: Aspergillus niger, Aspergillus flavus, Penicillium sp., Rhizopus sp. Brine shrimp larvae were used to determine the cytotoxicity activity of the selected methanolic plant extracts. Phytotoxicity screening was done by seed germination assay. The results indicated that all the selected plants possessed significant bioactivity and were not phytotoxic and cytotoxic.