Screening, production and optimization of Uricase from P. aeruginosa
C Selvaraj, P Thirumalai vasan
Uricase is very important for the determination of uric acid in biological fluids. Uricase has effective role in prophylaxis and treatment of tumor lysis hyperuricemia. In the present study, the uricase enzyme production was evaluated using P. aeruginosa. Enzyme production was studied for different pH, temperature, carbon, nitrogen source.In this current study the amount of enzyme produced was 73U/ml. The optimum temperature and pH was detected at 45°C and 5 for maximum uricase activity. The objective of the work was to develop a new method for screening of microbes for uricase production and estimation of uricase. This was achieved by utilizing the fact that uric acid dissolves on being acted upon by uricase higher production of uricase was obtained in this study. The proposed method is a novel, inexpensive, simple and sensitive technique for screening and estimation of uricase.