Immunosuppressive and antidiabetic activity of polyphenols from Musa paradisiaca
Abhijit Powar, Amit Gupta, Sanjay Kamble, Bharat Shinde
The objective of our study is to examine its immunosuppressive and antidiabetic potential of polyphenols extracted from the peels of Musa paradisiaca. In this study, indirect ELISA was performed in order to determine its immunogenicity against rubella vaccine antigen (Serum institute of India Limited, India) using variable doses of polyphenols (0.312 - 5 mg/ml; 100 µl). In addition, studies were also conducted on normal human whole blood samples in order to examine its proliferation against rubella vaccine antigen (1:500 dilution; 10 µl). Similarly, polyphenols of variable concentration were also tested in diabetic human whole blood samples and measured total cellular content. The results showed that polyphenols at higher doses (5 mg/ml; 100 µl) showed decline in antibody production (immunogenicity) and proliferation assay (rubella vaccine) in human whole blood samples. In contrast, polyphenols also showed reduction in total cellular content in diabetic human whole blood sample at a very low concentration (0.312 mg/ml; 100 µl). Overall, the results indicate that polyphenols extracted from the peels of Musa paradisiaca showed immunosuppressive and anti-diabetic effect.