Evaluation of antimicrobial activity of Senna (Senna italica Mill) plant and its synergistic effect with antibiotic drugs
Abdel Moneim E Sulieman, Ahmed A Alghamdi, Vajid N Veettil, Mohanad Abdelgadir, Nasir A Ibrahim
Senna (Senna italic Mill) one of the famous medicinal plants in many Arabic countries. In this study, the antibacterial activity of Senna and its synergistic effect with antibiotics were investigated. Different bacterial groups were used in this study including: Escherichia coli (E. coli), Klebsiella pneumoniae (K. pneumonia), Acinetobacter baumannii (A. baumannii), Pseudomonas aeruginosa (P. aeruginosa), Staphylococcus aureus (S. aureus) and Salmonella spp. The minimal inhibitory concentration (MIC) of the plant extracts against E. coli, S. aureus and P. aeruginosa were assessed using microdilution method. The synergistic effect between plants and extraction of antibiotics was evaluated using disk diffusion method. The results indicated that Senna plant has antibacterial properties against the tested bacteria with various degrees; this could be attributed to the plant active ingredients. Moreover, the zones of inhibition of all tested microorganisms resulted from plant extract-antibiotic combinations were greater than those resulted from antibiotics solely. The results also showed that methanolic extract of the plant has more inhibitory effect than that of the water extract. Further investigation of the plants extract to isolate and identify the active ingredients is recommended.