Isolation and charecterization lipase producing Bacillus flexus AW3(2), cultural conditions, nutritional requirements and some properties of crude enzyme
Vishal R Dhundale, Vijayshree M Hemke
Microbial culture dependent phenotypic characteristic were to study the lipase producing bacteria from the Lonar crater. Aerobic, haloalkaliphilic bacteria were isolated and characterized from sediment and water samples of Lonar Lake. Out of twenty nine, thirteen bacterial strains were selected on the basis of their lipolytic activities and subjected to physiological and standard biochemical characterization for their identification. Out of them strain AW3(2) was selected for 16S rDNA sequencing, production and, partial characterizations of lipase on the basis of their maximum lipolytic activity. A Gram positive bacterium was identified as Bacillus flexus by morphological, physiological, biochemical characterizations and 16S rDNA sequencing. Optimum enzyme activity was found to be at 70°C. The lipase was highly stable over a broad temperature from 40 to 90°C, and NaCl concentration 0.5-4M ranges, showing excellent thermostablity, and haloalkaline tolerant nature. The enzyme activity has enhanced by ZnSO2and NiCl2 indicating it was a metalloenzyme. Among the organic nitrogen sources, optimum lipase production was found to be in presence of ammonium nitrate. This is valuable information for enzyme production and optimization of lipase has a bright future towards the improvement and production of novel enzymes for entirely new areas of industrial and biotechnological applications involving molecular enzymology.
Vishal R Dhundale, Vijayshree M Hemke. Isolation and charecterization lipase producing Bacillus flexus AW3(2), cultural conditions, nutritional requirements and some properties of crude enzyme. European Journal of Biotechnology and Bioscience, Volume 5, Issue 2, 2017, Pages 41-46