European Journal of Biotechnology and Bioscience

Authors
Balamurugan TSB, Gunanithi M, Raja I, Dr. Vinothkumar D, Dr. Ramesh Babu NG, Sumathi S, Aneesh Nair, Florida Tilton

Abstract
A change in diet, lifestyle and exercise will help greatly in management of diabetes in the initial stages with lesser impact at later stages. Plant based products have been popular all over the world for centuries. In diabetics, some herbal alternatives are proven to provide symptomatic relief and assist in the prevention of the secondary complications of the disease. Some herbs have also been proven to the help in the regeneration of beta cells and in overcoming insulin resistance and to maintain normal blood sugar level. In the present study, an attempt has been made to investigate the antidiabetic activity of Trigonella foenum-graecum seeds, Acorus calamus rhizhome and Citrullus colocynthis fruit. Hexane, ethyl acetate and methanol extracts of three samples was assessed for phytochemical components qualitatively and quantitatively and for their antidiabetic activity. Methanol extract of Citrullus colocynthis shows the highest percentage of Glucose uptake activity and Alpha-amylase inhibition activity. The phytocomponents in the crude methanol extracts of the Citrullus colocynthis fruits were identified by GC-MS. The anti-diabetic compounds were sorted by the iGEMDOCK software docking. Then the compound binding was analysed at the active site of PPAR-gamma receptor using bioactive component.