European Journal of Biotechnology and Bioscience

Authors
Dr. N Lokeswari, Dr. Lenin Kumar B

Abstract
RP- HPLC is a simple, precise and simple technique which was used for qualitative assurance of Gallic acid from testa of Anacardium occidentale L., UV detection at 271nm was developed and validated. Separation was performed on a Phenomenax column (150 × 4.6 mm, 5 μm pore size) by using mobile phase, 80:20 ratiog of acetonitrile and water containing 0.01% v/v ortho phosphoric acid for the present study. For the separation and identification of gallic acid direct injection method has been performed in the HPLC technique. Studies, which activated the accurateness of the method, 99.63% recovery percentage was showed for this acid.