European Journal of Biotechnology and Bioscience

Authors
Ibrahim Toufik, Ilham Belkoura, Farhate Guenoun

Abstract
Somatic embryogenesis of Dahbia cultivar, a Moroccan olive tree, was induced on zygotic radicles explants according to a pre-established protocol for this olive cultivar. In fact, 19.6 % of explants turned into embryogenic callus which developed globular somatic embryos. These were then cultured under some conditions to induce their “germination”. The best response was occurred when globular somatic embryos were cultured on Olive Cyclic Embryogenesis free-hormone medium supplemented with sucrose (20g/L) and Activated Charcoal (1g/L) for three months under light (16h photoperiod). However, the conversion into plantlets remains hard to obtain; the best conversion percentage was 25%.