Improving germination conditions of somatic embryos derived from radicle explants of Moroccan olive cultivar Dahbia
Ibrahim Toufik, Ilham Belkoura, Farhate Guenoun
Somatic embryogenesis of Dahbia cultivar, a Moroccan olive tree, was induced on zygotic radicles explants according to a pre-established protocol for this olive cultivar. In fact, 19.6 % of explants turned into embryogenic callus which developed globular somatic embryos. These were then cultured under some conditions to induce their “germination”. The best response was occurred when globular somatic embryos were cultured on Olive Cyclic Embryogenesis free-hormone medium supplemented with sucrose (20g/L) and Activated Charcoal (1g/L) for three months under light (16h photoperiod). However, the conversion into plantlets remains hard to obtain; the best conversion percentage was 25%.
Ibrahim Toufik, Ilham Belkoura, Farhate Guenoun. Improving germination conditions of somatic embryos derived from radicle explants of Moroccan olive cultivar Dahbia. European Journal of Biotechnology and Bioscience, Volume 5, Issue 3, 2017, Pages 51-56