European Journal of Biotechnology and Bioscience

Authors
Dilek Keskin, Nur Ceyhan-Guvensen, Murat Uygun, Deniz Aktaş Uygun

Abstract
Propolis is a natural product widely consumed in folk medicine. The present study was carried out to investigate the antimicrobial activity of four different (Zonguldag, Cine, Cankırı and Ardahan) propolis regions of Anatolia. Antimicrobial activities of the propolis samples were investigated by the disc diffusion method. Although Ardahans’ propolis is the most effective (14/18), Cankırıs’ propolis is the least effective (4/18). The antioxidant activities of the extracts were tested by ferric thiocyanate (FTC) and 2,2-diphenyl-1-picrylrazy (DPPH) free radical scavenging methods. Zonguldag extract exhibited the greatest antioxidant activity with FTC method, whereas Cankiri extract exhibited the lowest IC50 value 5.69±0.49 (µg/mL) forDPPH radical scavenging activity. Total phenolic contents of the extracts were estimated by Folin-Ciocaltalteu method and Zonguldag extract was found to contain the highest amount (113.63±5.45 mgGAE/g) extract of phenolics. Ardahan extract contained highest both flavonoid content (20.07±1.59 RtE/g) and reducing power (52.60±0.45). Strong antioxidant, radical-scavenging and antimicrobial activities of Ardahan seemed to relate with high flavonoid contents and total polyphenol.