European Journal of Biotechnology and Bioscience

European Journal of Biotechnology and Bioscience


European Journal of Biotechnology and Bioscience
European Journal of Biotechnology and Bioscience
Vol. 5, Issue 6 (2017)

Screening of phosphate solubilizing bacterial isolates for improving growth of wheat


Ferdaouss El Habil-Addas, Saida Aarab, Abderrazak Rfaki, Amin Laglaoui, Mohammed Bakkali, Abdelhay Arakrak

With the aim to select beneficial bacteria exhibiting several plant growth promoting (PGP) traits and develop biofertilizers, the present study was conducted to characterize the native phosphate solubilizing bacteria (PSB) present in wheat rhizosphere in the Northwestern Morocco. A total of 122 rhizobacteria were isolated from the rhizosphere of two wheat varieties (Salama and Rajae) of which 34% was able to solubilizing tri-calcium phosphate (TCP) on Pikovskaya’s agar. On the basis of solubilization index (SI≥ 1.44), 10 rhizobacteria were selected and tested for hydrogen cyanide (HCN) production, quantitative assay of indole acetic acid (IAA) and siderophores production. Three isolates (Rs15, Rs19 and Rr1) were HCN producers. IAA production reached 31.08 mg/l by the isolate Rs19, whereas siderophores production was shown in Rs20, Rr1 and Rr3 isolates. Amount of solubilized phosphate ranged from 35.13 to 217.59 mg P L-1. Also, the effect of three isolates, Rs15, Rs19 and Rr1 on growth of wheat plants was investigated under culture chamber conditions. Significant increases were obtained for shoot length and dry weight of plants in presence of Rs15 and Rs19 as compared to the control. Analysis of 16S rDNA sequencing demonstrated that the isolates were closely related to Pantoea allii strain BD 390 (RS15), Stenotrophomonas maltophilia strain IAM 12423 (RS19) and Pseudomonas frederiksbergensis strain DSM 13022 (RR1).
Download  |  Pages : 07-11
How to cite this article:
Ferdaouss El Habil-Addas, Saida Aarab, Abderrazak Rfaki, Amin Laglaoui, Mohammed Bakkali, Abdelhay Arakrak. Screening of phosphate solubilizing bacterial isolates for improving growth of wheat. European Journal of Biotechnology and Bioscience, Volume 5, Issue 6, 2017, Pages 07-11
European Journal of Biotechnology and Bioscience