The aim of the present study was to investigate effect of pH, temperature and incubation period on dry cellular weight and lipid content of Rhodococcus opacus and Gordonia alkanivorans. The influence of various temperatures (20-50°C) on biomass yield and lipid accumulation were reported, Rhodococcus opacus showed significantly higher biomass yield 1.41±0.041gL-1 at 37°C. Rhodococcus opacus and Gordonia sp. revealed significantly (p≤0.05) higher lipid accumulation (14.52%, 13.84%) respectively at 37°C. Similarly effect of pH on oleaginous bacteria also studied that Gordonia sp. exhibited significantly (P≤0.05) higher biomass 2.77±0.034 gL-1 at pH 6 whereas Rhodococcus opacus showed significantly higher biomass 2.45±0.042gL-1 at pH 7. Effect of incubation period showed lipid content significantly (P≤0.05) enhanced up to 120 hrs in Rhodococcus opacus (13.78 %) whereas Gordonia alkanivorans yielded increased biomass (14.08%) upto 72 hrs in both species.
Kulvinder Bajwa, Narsi R Bishnoi, Anita Kirrolia, Jyoti Sharma, Saloni Gupta. Influence of temperature, pH variation and incubation period on single celled micro-organism. European Journal of Biotechnology and Bioscience, Volume 5, Issue 6, 2017, Pages 22-26