European Journal of Biotechnology and Bioscience

Authors
Silué Oumar, Kouassi Kan Modeste, Kouadio OI Kouadio Samuel, Koffi Kouablan Edmond, Sangaré Abdourahamane, Aké Séverin

Abstract
In the present study, shoot tip explants were used for shoot proliferation and rooting of two banana varieties (FHIA-21 and PITA-3). Shoot-tip cultures were maintained on Murashige and Skoog (MS) medium containing different levels of cytokinins combination [benzylaminopurine (BAP), kinetin (Kin) and 2-isopentenyl (2-iP)], auxins [indole acetic acid (IAA) and naphthaleneacetic acid (NAA)] and antioxidants [Silver nitrate (AgNO3), Citric acid (CA), Ascorbic acid (AA) and Cystein (C)] for shoot proliferation. The best response in terms of shoot number per explant was obtained from MS medium supplemented with BAP (3 mg/L) + Kin (0.25 or 0.5 mg/L) and with IAA (0.5 or 1 mg/L). The elongating shoots were cut to prepare further shoot proliferation. The addition of antioxydant such as Citric acid at 25 mg/L to the proliferation medium significantly improved the number and shoot length. Regenrated shoots were excised and rooted; the highest number and longest root were obtained with MS medium with 2-iP (1 mg/L) + NAA (0.5 mg/L + AgNO3 (10 or 15 mg/L) after three weeks. The rooted shoots were acclimatized and transfered to field conditions.