The multiplication of the argane tree by microcutting (Argania spinosa L. Skeels)
Meriyem Koufan, Ilham Belkoura, Tajlmolk Alaoui
Nowadays, In vitro technics and tools are the path to succeed fast, effective and large scale micropropagation in Argania spinosa. Indeed, within many species, the in vitro technics have been a success. Nevertheless, although the poor situation that argane tree suffers from, this tree hasn’t been undergone with many studies. In the current study, we emphasize on the use of axillary budding micropropagation in the population of OULAD AABIBOU in Morocco. We tested closely the effect of cutting location in the branch and mineral/hormonal medium in order to define optimal conditions for bud development. The medium Murashing and Skoog (1962), diluted by 50%, allowed the best bud development rate with 69, 82% for the intermediate location, and 25, 35% of the cutting shad been developed in leafy shoots. Furthermore, the rooting of young microcutting is developed in the medium MS/2 added by Naphthalene Acetic Acid (NAA) and Benzylaminopurine (BAP). In fact, using this medium, we have noticed the appearance of new root formation in the argane tree.
Meriyem Koufan, Ilham Belkoura, Tajlmolk Alaoui. The multiplication of the argane tree by microcutting (Argania spinosa L. Skeels). European Journal of Biotechnology and Bioscience, Volume 6, Issue 1, 2018, Pages 47-52