European Journal of Biotechnology and Bioscience

Authors
Bibek Khatiwada, Sobita Kunwar, Ashmita Dhakal, Ansu Joshi, Abdul Rehman Miya, Pradeep Subedi

Abstract
Natural products are considered as potential sources of pharmaceutical agents and/or as sources of lead compounds in drug development. In this study, two species of plant seeds were analyzed which are: Radish (Raphanus sativus L.) Rapeseed (Brassica napus L.) The present study was conducted to evaluate the the quantity of phytochemicals present, antioxidant activity, α-amylase inhibitory activity, and antibacterial activity of those two edible seeds. The plants extracts were obtained using methanol as a solvent. The total amount of phenolic content of different plant extracts was determined by Folin Ciocalteu method. The total flavonoids in the plant extract were estimated using the aluminum chloride colorimetry method. Antioxidant activity was analyzed by DPPH assay. Alpha-amylase inhibitory activity was determined by modified method described by the Worthington Enzyme Manual. The antibacterial activity was carried out by disc diffusion assay. The results revealed that the methanolic extract of rapeseed (1000μl) extract exhibited the highest radical scavenging activity with IC50 value of 323.30 mg/ml. Radish seed showed the highest alpha amylase inhibition activity with IC50 value of 1332.99 μg/ml. Both seeds showed significant antibacterial activity against six pathogenic bacterial species. Results obtained in this study indicated the promising medical potential of these two plant species.