European Journal of Biotechnology and Bioscience

Authors
Swapan K Tripathy, Digbijaya Swain, Dayanidhi Mishra, Arjun M Prusty, Suraj K Behera, P Tripathy, Bhaskar Chakma

Abstract
Anther culture generates haploid plants which can be converted to pure breeding double haploids by colchicine treatment. In rice, anther culture is highly species and genotype specific. O. glaberrima L. responds more to callus induction and plantlet regeneration than Oryza sativa genotypes. In Oryza sativa L., japonica sub-group responds better than indica. Within indica sub-group, there is wide variation in anther culture response. Genetic basis for callus induction and green plantlet regeneration is still unclear. This limits the use of this technique for routine use in rice breeding. In this pursuit, the authors presented a detailed review of the genetic control of callusing and plant regeneration response for successful use of the anther culture technique in rice breeding.