European Journal of Biotechnology and Bioscience

Authors
CMSC Chandrasekara, VGG Chandrajith, I Wickramasinghe

Abstract
The objective of the present study was to investigate the effect of enzyme pretreatment on tea extract from Black tea samples using Pectinex Ultra SP.L, and Viscozyme L enzymes. The effect of enzyme dosage was evaluated by analyzing the yield, color and turbidity of the tea extract obtained by carrying out an enzyme pretreatment with three different dosages. The antioxidant activity and Total polyphenol content of the tea extract were measured based on the DPPH radicals scavenging assay and Folin-ciocalteus (ISO 14502-1) method respectively. From the results of the experiment it was determined that there is no significant difference (p>0.05) in extractable solid yield and colour when the dosage was changed but there is a significant difference (p<0.05) turbidity when the dosage was changed. There is no significant difference (p>0.05) in the antioxidant activity and Total polyphenol content in enzyme treated tea extract and the control sample.