European Journal of Biotechnology and Bioscience

European Journal of Biotechnology and Bioscience


European Journal of Biotechnology and Bioscience
European Journal of Biotechnology and Bioscience
Vol. 6, Issue 5 (2018)

Effects of explant type and hormone combinations on callus induction at high production potential of phenolic compounds in Roselle (Hibiscus sabdariffa var. sabdariffa)


Kone Dramane, Yapo Sopie Edwige Salomé, ABEDA Zagadou Hermann, Kouadio Oi Kouadio Samuel, Koffi Edmond, Kouakou Tanoh Hilaire

Roselle is an annual tropical plant belonging to the family Malvaceae. It is highly prized by many populations around the world because of its richness in phenolic compounds. However, its production is sometimes slowed down by numerous biotic and abiotic constraints. To remedy this problem, several researchers have considered the production of phenolic compounds by in vitro culture from this plant. Our work consisted in putting in place in vitro techniques of induction of callus with high potential of production of phenolic compounds in this plant. Thus, cotyledon explants producing friable calli with good levels of phenolic compounds, after one month test were placed on different media. It was after three subcultures that cotyledon explants placed on M4 medium (MSB + 1mg/L 2,4-D + 1mg/L KIN) produced reddish coloring calluses with the highest phenolic content (31.72 mg/g).
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How to cite this article:
Kone Dramane, Yapo Sopie Edwige Salomé, ABEDA Zagadou Hermann, Kouadio Oi Kouadio Samuel, Koffi Edmond, Kouakou Tanoh Hilaire. Effects of explant type and hormone combinations on callus induction at high production potential of phenolic compounds in Roselle (Hibiscus sabdariffa var. sabdariffa). European Journal of Biotechnology and Bioscience, Volume 6, Issue 5, 2018, Pages 91-97
European Journal of Biotechnology and Bioscience