European Journal of Biotechnology and Bioscience

European Journal of Biotechnology and Bioscience


European Journal of Biotechnology and Bioscience
European Journal of Biotechnology and Bioscience
Vol. 6, Issue 6 (2018)

Assessment of genetic variability among various genotypes of critically endangered and medicinally important species: Chlorophytum borivilianum using RAPD markers


Shikha Desai, Parth Desai, Mounil Mankad, Ghanshyam Patil, MA Patel, Subhash Narayanan

In Ayurveda, safed musli is renowned as “Divya Aushad” and has been used in the preparation of over one hundred different ayurvedic medicines. Around the globe, wide genetic diversity of Chlorophytum species is present which can be base for selection, improvement and development of new varieties. DNA based molecular markers are best suited for systematic assessment of the genetic diversity, conservation and genetic improvement by breeding. The present investigation was carried out to find out the genetic diversity and patterns of relationships among different genotypes of Chlorophytum borivilianum using randomly amplified polymorphic DNA (RAPD) markers. Out of 80 primers screened, from four different series (A, L, N, O), 22 decamers which resulted with more than six scorable bands were selected. Total 277 loci were obtained, out of which 242 were polymorphic and 35 were monomorphic. Polymorphic information content (PIC) value varied from 0.736 to 0.937 with an average of 0.878, indicating a high level of polymorphism at all loci. According to Jaccard’s similarity coefficient, the lowest and highest similarities detected among eight different genotypes were 0.074 and 0.790, respectively. Unweighted pair group method with arithmetic mean (UPGMA) clustering resulted in two main clusters and similarity coefficientranged from 0.49 to 0.88. A huge genetic diversity was found among the various genotypes of C. borivilianum collected.
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How to cite this article:
Shikha Desai, Parth Desai, Mounil Mankad, Ghanshyam Patil, MA Patel, Subhash Narayanan. Assessment of genetic variability among various genotypes of critically endangered and medicinally important species: Chlorophytum borivilianum using RAPD markers. European Journal of Biotechnology and Bioscience, Volume 6, Issue 6, 2018, Pages 08-12
European Journal of Biotechnology and Bioscience