Identification of miRNA in Pan Troglodytes as genomics approach
Esha Dogra, Prashant Singh
The last decade has witnessed huge effort in the understanding of the various mechanisms by which small non coding RNAs regulate the various life processes associated with a cell. Such efforts have led to the identification of multiple small non coding RNAs such as miRNA, siRNA, tasiRNA, piRNA, rasiRNA and many more of them microRNAs with their ubiquitous presence in all domains of life has become the molecule of choice of many research efforts and mechanisms involving their biogenesis and final function in the cell has been elucidated through these works. However, still certain grey areas exist in our understanding regarding the phenomenon of regulation of these non-coding wonders. As we all are aware that most microRNAs are differentially regulated during the entire lifespan of an organism, it becomes very clear that certain regulatory proteins and their interacting partners play important role in the process. With this background this work was performed where the precursor sequences of microRNAs of Pan troglodytes were considered as query and through position specific weight matrix evaluation and subsequent validation a large number of RNA regulatory elements were identified in those sequences.