European Journal of Biotechnology and Bioscience

Authors
Ukpanukpong RU, Eban LK, FD Adebiyi, Aigbadumah PO, Yusuff AA, Akpan UE

Abstract
The ameliorative antioxidant effect of vitamin C, E and garlic on tramadol induced toxicity was studied in wistar rats. Thirty five (35) rats were used and divided into five study groups with seven rats each. There was significant decrease in the body weight of the negative control group compared with the positive control group. Also, there was a significant increase in the body weight indices of antioxidant vitamins C, E and garlic treated groups when compared with the negative control at (P < 0.05). The organ weight of the liver in the negative control group increased significantly when compared to the weight of the liver in the positive control group. This reveals the metabolic changes in the liver as the site of drug metabolism which resulted in the inflammation of the liver. The weights of the heart and kidney also showed similar differences in the graphs. There was significant decrease in the Na⁺, K⁺, level of the negative control compared to the positive control group at (P<0.05). The total level of antioxidants vitamin C, E and garlic increased significantly when compared to the negative control group (P<0.05). The level of K⁺ in the negative control group reduced significantly when compared to the positive control group at (P<0.05) but HCO₃^- increased significantly in the negative control compared to the positive control at (P<0.05), in other groups (TmVC, TmVE, TmG), HCO₃^- elevated significantly compared to the control group at (P<0.05). The serum activities of female hormones; LH and Estrogen group showed significant decrease in the negative control group at (P<0.05) when compared to the positive control group at (P<0.05) but in the TmVC, TmVE, TmG group, LH and estrogen showed significant increase but not as the positive control group.