European Journal of Biotechnology and Bioscience

European Journal of Biotechnology and Bioscience


European Journal of Biotechnology and Bioscience
European Journal of Biotechnology and Bioscience
Vol. 7, Issue 6 (2019)

Sequestration and purification of laccase, catalase and lipase from oyster mushroom (Pleurotus florida) compost waste and its application studies


Prabhu N, Gajendran T, Karthika Devi S, Kavitha K, Shalini K, Vaishali S

Discarded oyster mushroom (Pleurotus florida) bed as tremendous source for isolation of industrial significant enzymes such as Amylase, Cellulase, Protease, Laccase, Lipase, Catalase, Xylanase, Lignin peroxidase, manganese peroxidase and pectinase. This study was conducted in order to achieve efficient extraction of lignocellulolytic enzymes Laccase (EC 1.10.3.2), Lipase (EC 3.1.1.3) and Catalase from spent oyster mushroom (Pleurotus florida) compost waste. Optimal enzyme recovery was achieved when spent oyster mushroom (Pleurotus florida) compost wastes were homogenized in 250ml of 50mM sodium acetate (pH 5.4) buffer at 5 ˚C for 2hr. The enzyme laccase released from oyster mushroom (Pleurotus florida) compost waste were showed activity of 0.962 U/ml. These were utilized in various industrial and environmental applications such as dye decolorization from textile industry waste water and Biosurfactant production. The synthetic dye Bromophenol blue and Cango red were showed high decolorization percentage (93.4% and 44%) in the presence of Pleurotus florida compost waste within 24 hrs. The crude extract of Pleurotus florida compost waste was successfully used in various other bioremediation applications.
Download  |  Pages : 23-29
How to cite this article:
Prabhu N, Gajendran T, Karthika Devi S, Kavitha K, Shalini K, Vaishali S. Sequestration and purification of laccase, catalase and lipase from oyster mushroom (Pleurotus florida) compost waste and its application studies. European Journal of Biotechnology and Bioscience, Volume 7, Issue 6, 2019, Pages 23-29
European Journal of Biotechnology and Bioscience