European Journal of Biotechnology and Bioscience

European Journal of Biotechnology and Bioscience


European Journal of Biotechnology and Bioscience
European Journal of Biotechnology and Bioscience
Vol. 7, Issue 6 (2019)

Comparison studies on lipase production using immobilized Exiguobacterium aestuari by repeated batch bioreactor


Prabhu N, Gajendran T, Kokilavani, Dhanapriya S, Preetha K

Enzymes play a pivotal role in a number of industries. In recent years there has been potential increase in the use of lipase as industrial catalysts. Exiguobacterium aestuari has been found to be a potent producer of lipase. This study is focused at the optimization, extraction and purification of Lipase from the organism. Maximum activity was obtained at pH 7.0 and temperature 370C. Carbon source glucose gave a maximum activity. A medium containing yeast extract and 1% olive oil also generated maximum activity. The lipase enzyme was precipitated using Ammonium sulphate and purified by using a dialysis membrane. The activity of purified enzyme was increased thre-fold compared to crude enzyme. The enzyme isolated was subjected to characterization studies. Solid state fermentation of lipase was carried out in coconut, sesame and ground nut oil cakes. Maximum activity of lipase was sesame oil cake as the substrate.
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How to cite this article:
Prabhu N, Gajendran T, Kokilavani, Dhanapriya S, Preetha K. Comparison studies on lipase production using immobilized Exiguobacterium aestuari by repeated batch bioreactor. European Journal of Biotechnology and Bioscience, Volume 7, Issue 6, 2019, Pages 30-37
European Journal of Biotechnology and Bioscience