Determination of genetic diversity of Gluconacebacter hansenii with RAPD and ERIC-PCR
Bahadır Törün, Esin Poyrazoğlu Çoban, Bülent Bozdogan, H Halil Bıyık
Cellulose production is typical for Gluconacebacter species. Bacterial cellulose is mainly used in paper and textile industry, high-performance speaker diaphragms, dietary foods, artificial skin, paint thinner, medical underwear, wound covering material, cosmetic makeup pads and facemask making. In this study, RAPD-PCR and ERIC-PCR method, which are PCR-based methods, was used to determine the genetic diversity of Gluconacebacter hansenii. Nine Gluconacebacter hansenii strains, ten RAPD primers and one pair of ERIC primers were used. The RAPD-PCR method produced 106 bands. Out of 106 bands that obtained from RAPD primers, 86 of them were polymorphic which indicates 81.13% polymorphism ratio. ERIC primers have also shown high polymorphism and distinguished species on strain level. As a result of study, the similarities of the strains formed by deriving from the data obtained are seen. Taking notice of the calculated polymorphism rate, the genetic diversity of the strains appears to be at the expected level.
Bahadır Törün, Esin Poyrazoğlu Çoban, Bülent Bozdogan, H Halil Bıyık. Determination of genetic diversity of Gluconacebacter hansenii with RAPD and ERIC-PCR. European Journal of Biotechnology and Bioscience, Volume 7, Issue 6, 2019, Pages 49-54