Comparative effect of various extracts of Morinda morindoides (Baker) Milne-redhead (Rubiaceae) on the in vitro growth of candida albicans and Trichophyton rubrum
Gboko Abiba Ouattara, Kamagate Tidiane, Kokora Aya Philomene, Touré Abdoulaye, Coulibaly Adama
Morinda morindoides is a medicinal herbal traditionally used to fight against infectious diseases such as opportunistic mycoses and infantile diarrhea in Ivory Coast. The aim of present study is to compare the antifungal activities of three extracts of Morinda morindoides leaves: total aqueous extract (ATE), hydro-ethanolic extract (HE) and total protein extract (TP)) on the in vitro growth of Candida albicans and Trichophyton rubrum. The both dilution method in bent tube solid medium was used to evaluate antimicrobial activity of various extracts and to determinate antimicrobial parameters. The tests are realized at different concentrations from 4.69 to 300 mg / ml for the ATE and from 0.78 to 50 mg / ml for the HE and TP. After incubation time, the strain tested is sensitive to HE and ATE extracts in a dose-response relationship. The results reveal that only the TP has no effect on the growth of the two fungal sprouts. However the HE shows the highest antimicrobial activity with MFC = 25 mg / ml and IC50 = 7.25mg / ml for Candida albicans and MFC = 25 mg / ml, IC50 = 13.75mg / ml for Trichophyton rubrum followed by ATE. These results could justify the traditional use of Morinda morindoides against cutaneous fungal infections.
Gboko Abiba Ouattara, Kamagate Tidiane, Kokora Aya Philomene, Touré Abdoulaye, Coulibaly Adama. Comparative effect of various extracts of Morinda morindoides (Baker) Milne-redhead (Rubiaceae) on the in vitro growth of candida albicans and Trichophyton rubrum. European Journal of Biotechnology and Bioscience, Volume 7, Issue 6, 2019, Pages 84-88