Ectomycorrhizal synthesis of Lactarius sanguifluus (Paulet) Fr. with Abies pindrow Royle Ex D. don
Shiv Kumar, Anand Sagar, Amit Kumar Sehgal
This study was aimed to perform in vitro mycorrhizal synthesis between Abies pindrow and Lactarius sanguifluus was achieved. A. pindrow seedlings inoculated with mycelial culture of L. sanguifluus resulted in the formation of short, branched lateral roots which ultimately form ectotrophic mycorrhizae. Synthesized mycorrhizae were light brown to pale yellow in colour. The transverse sections of the synthesized roots showed a typical ectomycorrhizal anatomy. The anatomical structure of mycorrhiza revealed the presence of thick fungal mantle and well developed “Hartig net”. Pure culture of L. sanguifluus was reisolated from both vermiculite peat moss mixture and synthesized ectomycorrhizae. These were compared with the original culture isolated from the fruiting bodies of L. sanguifluus and were found to have same cultural characteristics, thus confirming the symbiotic association.
Shiv Kumar, Anand Sagar, Amit Kumar Sehgal. Ectomycorrhizal synthesis of Lactarius sanguifluus (Paulet) Fr. with Abies pindrow Royle Ex D. don. European Journal of Biotechnology and Bioscience, Volume 7, Issue 6, 2019, Pages 89-92