European Journal of Biotechnology and Bioscience

Authors
Tapash Kumar Bhowmik, Md Mahbubur Rahman

Abstract
A protocol for in vitro germination and micropropagation of Cymbidium cyperifolium Wall. is an ornamental orchid species of Bangladesh, has been developed and PM basal medium was best for seeds germination than MS, KC, MVW media correspondingly. Sucrose was better for germination as source of carbohydrate than glucose and lactose. Maximum elongation of seedlings was recorded on liquid MS medium with 0.5 mg/l IAA + 1.0 mg/l BAP (4.48 ± 0.13cm) followed by agar solidified MS + 0.5 mg/l NAA + 1.0 mg/l BAP (3.31 ± 0.14cm) medium. Nodal segments directly produced highest average number of multiple shoot buds via organogenesis sprouted on MS + 1.0 mg/l IBA + 2.0 mg/l BAP (5.89 ± 0.35/segment) medium followed by MS + 1.0 mg/l NAA + 2.0 mg/l BAP (5.43 ± 0.36/segment) medium. Leaf segments underwent proliferation via embryogenesis and produced maximum percent of greenish PLBs on MS medium + 1.0 mg/l IBA + 2.0 mg/l Kn followed by MS + 1.0 mg/l Pic + 2.0 mg/l Kn. Highest mean increased individual shoot bud length was achieved on liquid MS with 0.5 mg/l IAA + 1.0 mg/l BAP (3.57 ± 0.15cm) followed by agar solidified MS with 1.0 mg/l Pic + 1.0 mg/l BAP (3.33 ± 0.12cm) medium. Full strength MS media supplemented with 0.5 mg/l NAA was the ideal condition for root formation followed by half strength MS0 medium. The highest number of SPSs formation took place in liquid MS + 0.5 mg/l IAA + 1.0 mg/l BAP followed by agar solidified PM + 1.0 mg/l Pic + 1.0 mg/l BAP medium. Rooted plantlets were able to grow in ex vitro condition after a short period of acclimatization.