European Journal of Biotechnology and Bioscience

Authors
Tapash Kumar Bhowmik, Md Mahbubur Rahman

Abstract
Habenaria digitata Lindl. is a indigenous terrestrial orchid, infrequently distributed in subtropical mountains and rainforests area of Jaflong, Sylhet, Bangladesh. The seeds of this orchid can be germinated asymbiotically in vitro for rapid propagation. In this study, half and full strength KC, MS, PM and VW basal media with or without PGRs combination showed various responses. Amongst four basal media used, MS medium with PGRs combination gave the maximum percentage response (86.87%) followed by PGRs supplemented PM (80.00%), VW (53.34%) and KC (46.67%) media. Minimum percentage of seed germination was observed on full strength PGRs free KC (26.67%) medium. Half strength PGRs free KC and VW media did not show any responses for germination of this orchid species. Minimum time needed for initiation of germination in full strength PGRs supplemented MS (8.07 ± 0.13^b weeks) medium, whereas, maximum time required (15.13 ± 0.22ⁱ) in PGRs free full strength KC medium.