Genotypic detection of virulence factors from different types of E.coli isolated from diarrheic Camel Calves in Sudan
Muna E Ahmed, Sabiel YA, Abeer AM, Manal H Salih, Marmar A El Siddig, Twasul M Mohammed, Abeer M Abass, El-gadal AA, Hind EA
The study conducted to isolate and characterized E. coli associated with diarrhea in camel calves in Sudan. Conventional bacteriological methods, Api 20 E. strips were used for primary identification of E. coli and polymerase chain reaction technique was used for molecular characterization. Two hundred fecal samples taken from calve camels suffering diarrhea were collected from Gezira, West Kordofan and North Kordofan States. The collected samples subjected to bacteriological examination for isolation and identification of E. coli. Sixty Escherichia coli were isolated and characterized (30%). The characterized E. coli were tested for the presence of virulence genes using multiplex PCR for fimbriae F41, F5, eae, heat-labile (LT) and heat-stable (STa), enterotoxins Stx1, saa and hly. 72 virulent genes were detected from 60 isolates carried genes for at least one of the virulence factors tested. The gene encoding for eae was the most prevalent 20(27.78%) followed by those encoding for stx 15 (20.83%), f5 14(19.44%), 41 7(9.74%), LT 6(8.34%), sta 5(6.94%), saa 4(5.55%) and hly 1(1.38%). Different pathogenic E.coli from camel calves were detected containingvarious pathogroup this indicated the important role of E.coli in contamination of environment. The study concluded that, camel calves considered as a reservoir for many pathogens carrying resistance genes as E. coli.
Muna E Ahmed, Sabiel YA, Abeer AM, Manal H Salih, Marmar A El Siddig, Twasul M Mohammed, Abeer M Abass, El-gadal AA, Hind EA. Genotypic detection of virulence factors from different types of E. coli isolated from diarrheic Camel Calves in Sudan. European Journal of Biotechnology and Bioscience, Volume 8, Issue 6, 2020, Pages 01-05