Supplementary diet of Nigella sativa on immune response and diseases resistance in Oreochromis mossambicus
A Jeyaprabha, Alageswari, S Durgadevi, R Kavimani, S Subeena Begum
The present work was designed to evaluate the effect of Nigella sativa oral supplementation with the basal diet on the Immunological Assays and Disease resistance test in Oreochromis mossambicus. A total of 70 juvenile’s tilapia were taken randomly and divided into four experimental units (10 fishes per unit) with three replicates. Fishes were given four different feed containing basal diet for E0 (Control), the experimental group N.sativa E1= basal diet supplemented with 0.05% N.sativa powder. The experimental group N.sativa E2= basal diet supplemented with 0.5% N.sativa powder. The experimental group N.sativa E3= basal diet supplemented with 5.0% N.sativa powder. Experimental period was 60 days. At the end of the trial immune response such as White blood cells (WBC), Red blood cells (RBC), Skin Mucus Protein, Haemoglobin content, Serum Protein, Serum Lysozyme Activity, Phagocyte activity and Disease Resistance test monitored in all group of fishes. The Immune response enhanced in E1, E2, and E3 when compared with E0 Control. Moreover all Immunological assays and Disease resistance has significantly enhanced in E2 experimental diet (0.5 %) when compare with control diet. White Blood cells (29.34 ± 0.88), Red Blood cells (1.57 ± 0.05), Haemoglobin content (8.57 ± 0.25), Skin Mucus Protein (22.02 ± 0.66), Serum Protein (3.82 ± 0.01), Serum Lysozyme (634 ± 1.90), Phagocyte Activity (36.4 ± 1.09) has observed better Immune response in O.mossambicus. The disease resistance (Relative percent survival) has also monitored better in 0.5% experimental diet fed fish group. So, it is concluded that Nigella sativa (0.5%) can be supplemented with basal diet to enhance immune status and improve disease resistance in Oreochrombis mossambicus.
A Jeyaprabha, Alageswari, S Durgadevi, R Kavimani, S Subeena Begum. Supplementary diet of Nigella sativa on immune response and diseases resistance in Oreochromis mossambicus. European Journal of Biotechnology and Bioscience, Volume 8, Issue 6, 2020, Pages 25-30