European Journal of Biotechnology and Bioscience

European Journal of Biotechnology and Bioscience


European Journal of Biotechnology and Bioscience
European Journal of Biotechnology and Bioscience
Vol. 9, Issue 1 (2021)

Phytochemical analysis and antimicrobial activity of Hibiscus Rosa Sinensis


Kumari Priya, HP Sharma

Hibiscus rosa sinensis is a plant well known for its various medicinal properties. The aim of this research work was to find out the antibacterial activity and phytochemical analysis of whole plant extracts (root, flower, and leaves). In the present investigation phytochemicals analysis of various parts of the plants were done which shows presence of these phytochemicals alkaloids, terpenoids, tannins, flavonoids, saponins, phenolic compounds, carbohydrate, reducing sugar and triterpenoids. Further saponin content estimation revealed lowest amount of saponin in 2.5mg/g in HRlf followed by 3.1mg/g in HRfr and highest amount 4.3mg/g in HRrt. Moreover, antibacterial activities of different plant extracts were evaluated on gram negative bacteria S.aureus by Agar well-diffusion method in different concentration of 20, 50 and 100μg/ml and zone of inhibition were measured. The maximum zone of inhibition shown in concentration of 100μg/ml methanolic leaf extract of H.rosa sinensis (29.00+2.81mm), followed by methanolic flower extract (17.00+0.99mm), followed by aqueous flower extract(14.00+0.90mm) followed by methanolic root extract (13.00+0.75mm). MeOH extract of leafs exhibits highest antibacterial activity among tested ones. Current studies have shown that the H.rosa sinensis plant contains significant antimicrobial activities in the MeOH extracts. The antibacterial activities of H.rosa are may be due to presence of saponin, tannins, and flavonoids.
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How to cite this article:
Kumari Priya, HP Sharma. Phytochemical analysis and antimicrobial activity of Hibiscus Rosa Sinensis. European Journal of Biotechnology and Bioscience, Volume 9, Issue 1, 2021, Pages 21-26
European Journal of Biotechnology and Bioscience