in vitro cloning of Guadua angustifolia kunth–an exotic, fast growing and industrially important bamboo species

Somashekar PV; Rathore TS; Almas Khannam

in vitro cloning of Guadua angustifolia kunth–an exotic, fast growing and industrially important bamboo species

Authors
Somashekar PV, Rathore TS, Almas Khannam

Abstract
Micropropagation studies were conducted on the effect of media, growth hormones for shoot initiation, shoot multiplication, in vitro and ex vitro rooting. Nodal explants from mature culms of Guadua angustifolia were used for shoot induction. Maximum of 4-5 shoots exhibited on MS liquid medium fortified with additives + NAA 0.25 mg/l + BAP 5.0mg/l. In vitro differentiated shoots were further multiplied on MS liquid medium supplemented with additives + NAA 0.25 mg/l + BAP 2.5 – 5.0 mg/l. The Shoot multiplication rate was 4-5 folds within a 4 week period. Liquid medium proved better than agar gel medium for shoot initiation and multiplication. In vitro raised propagules (2-3 shoots/clump) exhibited 100% in vitro rooting on MS/4 basal salts medium fortified with NAA 1.0 mg/l within a 4 week period. For ex vitro rooting, in vitro shoots were pulse treated with NAA (1000 ppm) for 5 mins and transplanted in the sand as rooting media. Pulse treated shoot clumps exhibited high frequency (95.55%) root induction. The survival rate of in vitro plants in the field was 100 percent at six months from planting.

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